The Wilbur-Cruce Mustang (also known as the 'Cruce Colonial Spanish Mission Horse') is a threatened strain of the Spanish Mustang, which is found in Arizona. It is considered to be a direct descendant of Spanish horses, who were brought to the Americas during the 17th and 18th centuries, and who were bred by Father Francisco Eusibio Kino.

In the 19th century, a man named Dr. Ruben Wilbur established a cattle farm in Arizona, and bought some of the descendants of Father Kino's horses. He kept these horses, now known as Wilbur-Cruce horses, in semi-feral conditions, meaning that he allowed them to live and breed in the pastures, and trained and rode them when necessary. This practice lasted until the late 20th century, when the Wilbur-Cruce Mustangs were genetically identified as non-feral, yet pure, descendants of the Spanish horses, and were moved to a protected herd-management area.

Wilbur-Cruce Mustangs are smart horses with good endurance. They come in the colors of chestnut, bay, grey, and black, and might have an overo or sabino coat. Cream-diluted individuals exist but are extremely rare. Wilbur-Cruce Mustangs stand between 14hh and 15.2hh.